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When it comes to the baking questions I get asked most frequently, substitutions is definitely one of the major themes.
Whether it’s due to food allergies or intolerances or lack of availability or convenience, I know it’s not always possible to have or use every ingredient on hand for a recipe you might want to try out.
Answering specific substitution questions becomes challenging, however, when I haven’t tested a particular substitution for a particular recipe. It makes me uncomfortable to answer these types of questions because I simply don’t have 100% confidence to say whether a substitution will definitely work or not.
That’s actually why I created my Baking Substitutions guide. It’s a super handy resource for those times when you don’t have a particular ingredient on hand; however, even though it took me weeks to make that guide, I still haven’t tested every substitution instance or done in-depth side-by-side comparisons.
That’s also why I love to do these kinds of baking experiments. It’s so hard to judge the taste and texture of a substitution without testing it directly next to the original, or the “control.” And since buttermilk is an ingredient I get asked about a lot, I thought a Buttermilk 101 post would be a perfect reference for your own baking experiments!
If you like these kinds of posts then you’ll LOVE The Magic of Baking online course + community. It dives deep into baking science in a fun, visual, & approachable way so you can enter the kitchen with complete confidence.
Tools and Ingredients Used
I made every effort to replicate each batch as perfectly as possible, using the same exact tools and ingredients whenever applicable. I used a kitchen scale to measure ingredients to ensure 100% accuracy and used an oven thermometer to gauge exact baking temperatures. Each batch was baked for exactly 13 minutes.
Here are the base ingredients and tools I used for reference:
- Gold Medal Bleached All-Purpose Flour
- Simple Truth Organic Butter
- Clabber Girl Double Acting Baking Powder
- Liquid measuring cup
- OXO Steel Blade Pastry Blender
- Nordic Ware Natural Aluminum Sheet Pans
- Unbleached Parchment Paper
What is Buttermilk?
- Essentially, buttermilk is fermented milk.
- Originally, buttermilk was the leftover liquid produced following the churning of cream into butter. When butter was made at home, that leftover liquid was left out overnight to ferment.
- Modern buttermilk is now made by adding lactic acid-producing bacteria to milk (usually low-fat milk) to “culture” it in a controlled environment.
- The acid affects the casein proteins’ ability to come together by lowering the pH of the milk, and that’s what produces the thick texture of buttermilk.
- Buttermilk is used primarily for its pleasant tangy flavor, but it can also help tenderize and leaven certain baked goods.
Testing Buttermilk Substitutes
When it comes to buttermilk, the most common question I get is how to best substitute it.
I get it, most of us don’t have “real” buttermilk in our fridge all of the time, and running back to the grocery store for one or two things can be such a hassle. However, since I know from all of my Ultimate Guides, one small change in a baking recipe can have significant results. So I decided to put the most common buttermilk substitutions to the test.
Control Recipe
1 cup store-bought buttermilk
All of the biscuits I baked were based on my go-to biscuit recipe found at the bottom of this post. I re-made the same recipe each time, simply changing out the buttermilk for each batch.
These control “real” buttermilk biscuits were exactly as I anticipated: tangy, buttery, tall, appealingly craggy, with a coarse open crumb – as traditional buttermilk biscuits should be. These represent why I love buttermilk so much! The flavor was fantastic.
DIY Buttermilk + How to Make Buttermilk
1 scant cup whole milk + 1 tablespoon distilled white vinegar
I combined the two and let sit for 5 minutes before mixing into the dough. The mixture was definitely thinner than actual buttermilk, and the dough was much wetter and stickier, which made it more difficult to work with.
I was super excited to do a direct taste test with these “homemade buttermilk” biscuits compared to the “real” buttermilk biscuits. When I pulled these out of the oven, I was impressed by the lovely brown color they had developed. Unfortunately, the DIY buttermilk noticeably lacked the complex tangy flavor of the control buttermilk biscuits and instead contained a sharp sourness. They also had a finer crumb which I found to be more similar to store-bought or fast food biscuits.
Buttermilk Powder
1 cup water + 4 tablespoons buttermilk powder
I used the Saco brand of cultured buttermilk powder that I found at my grocery store and followed the substitution directions listed on the packaging. I used filtered water. The package’s instructions directed me to blend the powder into the dry ingredients, which is what I did.
Where the control biscuits were pleasantly tangy, these biscuits were strangely sour. They also baked up flatter than any of the other batches. The package directions also said to store the powder in the refrigerator after opening, which to me doesn’t make it all that much more convenient than actual buttermilk, especially for the amount of flavor you’re giving up to use this.
Whole Milk
1 cup plain milk
These regular milk biscuits turned out craggy and nicely golden, and had an intense buttery flavor. They tasted the most buttery of all the batches, which makes sense considering there was no excess acidity here to cut through the fat and richness. These also had a finer crumb similar to the DIY buttermilk biscuits, which was interesting. The finer crumb reminded me again of fast food biscuits or premade store-bought biscuits.
Plain Yogurt
1 cup thinned plain yogurt
I thinned the yogurt considerably with water until it was pourable, before adding into the dough. Surprisingly, these biscuits ended up being my favorite behind the control batch. They had good flavor and a tall, even shape. The only downside was that, like the DIY buttermilk biscuits, these had a finer texture similar to fast food biscuits (think KFC). However, because I know some people enjoy that texture, using plain yogurt might be a great option for you! You could even try a similar experiment with sour cream.
Vegan/Dairy-Free Buttermilk Substitute
1 scant cup full-fat canned coconut milk + 1 tablespoon distilled vinegar
I combined the two and let sit for 5 minutes before mixing into the dough. I did *not* substitute the dairy butter in this batch because I wanted a direct comparison against the control batch and because this post is all about buttermilk. Check out my biscuit comparison with butter vs. shortening here if you’re interested.
I was curious to see if I could make a dairy-free substitute this way, thinking that coconut milk is rich and thick, unlike other non-dairy milks like almond milk or soy milk. The consistency ended up being very similar to real buttermilk, which made me excited. However, the resulting biscuits had a completely different flavor profile from all of the other biscuits and were way more crumbly. These were definitely not my favorite.
Final Comparison
It’s funny because I feel like the biscuits appear to look very similar, but there were definitely noticeable differences in taste and texture.
Looks like I’ll be sticking with store-bought real buttermilk for the foreseeable future, since homemade buttermilk substitutes just didn’t yield the same results.
I also have tested a standard muffin recipe with whole milk vs. buttermilk to see how it impacts a different type of recipe:
The buttermilk muffins had a more craggly open-crumb texture than the whole milk muffins and were SUPER moist and flavorful. I absolutely loved this batch and don’t think I’ll make muffins without buttermilk again. You can see my full Ultimate Guide to Muffins post here.
So as you can see, real buttermilk is the BEST choice. But since I know it’s not always easy to keep buttermilk on hand, I created the below video on How to Freeze Buttermilk so you always have some ready to use (and so you don’t waste any).
How to Freeze Buttermilk
More Baking Science Articles:
- Cake Flour 101
- Best Baking Pans
- Baking Soda vs. Baking Powder
- How to Measure Flour the RIGHT Way
- Ultimate Guide to Chocolate Chip Cookies
Recipes with Buttermilk:
- Blueberry Cobbler
- Buttermilk Pancakes
- Chocolate Chip Muffins
- Best Homemade Waffles
- Peach Cobbler Recipe
If you need more help with baking substitutions and swaps, check out my free guide:
Basic Buttermilk Biscuits
Ingredients
- 2 cups (254 grams) all-purpose flour, plus more for dusting
- 2 teaspoons granulated sugar
- 1 tablespoon baking powder
- 1/4 teaspoon baking soda
- 1 teaspoon fine salt
- 6 tablespoons (85 grams) unsalted butter, very cold
- 1 cup buttermilk, chilled
Instructions
- Preheat the oven to 450°F.
- In a large bowl, combine the flour, sugar, baking powder, baking soda and salt. Using your fingertips or a pastry blender, cut the butter into the flour mixture until it resembles coarse meal. You can also do this in a food processor. The faster you do this the better, you want the fat to remain cold. Stir in the buttermilk until just combined. DO NOT overmix, the dough will be slightly sticky.
- Turn the dough out onto a floured surface and dust the dough with flour. Gently pat the dough out until it’s a 1/2-inch in thickness. Use a 2-inch round biscuit cutter to push straight down through the dough to cut out circles, try not to twist the cutter. Place the biscuits on the baking sheet, spacing 2-inches apart. Reform the scrap dough into 1/2-inch thickness, being sure to work with it as little as possible, before cutting out more circles.
- Bake the biscuits until golden brown, about 12 to 15 minutes.
Buttermilk 101 Article Credits:
- Written by Tessa Arias
- Edited by Jessie Bruce, Master’s of Public Health Nutrition and Dietetics Candidate at UC Berkeley
Sources:
- Amendola, J. (2002). Understanding Baking: The Art and Science of Baking (3rd Edition). Wiley Global Education US. https://bookshelf.vitalsource.com/books/9781118558713
- Figoni, Paula. How Baking Works: Exploring the Fundamentals of Baking Science, 3rd Edition. 3rd ed., John Wiley and Sons, 2010.
- McWilliams, M. (2016). Foods (8th Edition). Pearson Education (US). https://bookshelf.vitalsource.com/books/9780134204666
I’m not an expert, but I believe when you make substitutions either with or for buttermilk, you have to adjust for the change in acidity or your results will not be good. Baking soda is alkaline and will react with the acidity in the buttermilk to make the carbon dioxide bubbles that raise the dough. Baking powder contains an acid along with baking soda, so it works to raise the dough all by itself. If you substitute buttermilk for regular milk, the extra acid will overwhelm the baking powder and it will not raise well, so you have to reduce or eliminate the baking powder and add some baking soda. The same is true in reverse. If a recipe is formulated for buttermilk it probably calls for baking soda. If you substitute regular milk there will not be enough acidity to react with the baking soda, so you need to replace it with more baking powder. I suspect that’s why some of your trials resulted in a finer crumb, because they weren’t able to raise fully.
Have you used buttermilk past the due date? It doesn’t seem to smell much different.
I’ve only ever tried vinegar or lemon in milk, so i’m curious about the yogurt option. How much yogurt and water would I need to make 1 cup of buttermilk substitute?
I was very interested in reading this article as I’ve always made my own buttermilk (I don’t use it often enough to keep a carton in the fridge). But I’m wondering if lemon juice would have a better flavor than vinegar. The way you describe the taste is exactly how I imagine DIY with vinegar would taste (I use lemon juice myself). Have you ever done a side-by-side comparison with the two methods?
Hardly any difference, Katie. The taste AND texture of *any* DIY version vs the real thing are very different (even if directly sipped for taste side-by-side without baking).
This was so helpful! Thanks Tessa! Also, I love how you suggested to first try be recipe exactly as written so you know what it’s supposed to taste like. Awesome advice. Thanks again!
I’m so glad you thought so, Nathalia!
This was so interesting! I love these types of articles! Keep them coming!
I enjoy these guides so much….thank you! I’ve read lemon juice (in place of vinegar) and milk is another common buttermilk substitution. Have you ever tried it? Would it be similar to the milk/vinegar sub?
Yes, Sandra, that’s basically the same as the vinegar version.
These tests are very useful, thank you for conducting them. When we live outside the US, some ingredients are hard to find. Here in Brazil we do not have buttermilk, I’ve always used the milk+vinegar substitution. I’ve never tried the yogurt substitution, I’ll give it a try. But I was wondering if thinning it with low fat or whole milk would be better than water. Have you ever tried it?
I found water worked best to thin it to the right consistency.
Katie can u not sub. Sour cream for buttermilk
Such a valuable resource, have always needed to know more about buttermilk as an ingredient but don’t use it enough to really get a handle on it, certainly not enough to have done all of the research in this article, so thank you for this valuable info!
Amazing! Send your resume to America’s Test Kitchen. The work that you put into this for all of us is very much appreciated. It is so thorough, easy to understand, and beautifully displayed.
Thanks, Tessa!
Awe, thanks so much Amanda!
This is a particularly useful guide. I’m disappointed that the buttermilk powder didn’t yield good results, that would have been so handy! As I’ve only seen buttermilk in quarts, do you know if it freezes well? Also, thanks for including coconut milk, my son is vegan, so that would have been a waste of my time and money.
I know :/ Yes, buttermilk does freeze well! You can freeze it in ice cube trays. You’ll want to vigorously whisk or blend the buttermilk after thawing before using because it will separate.
Hi Tessa, this is such a wonderful guide. I often won’t even consider recipes calling for buttermilk but now I can give them a shot! With your yogurt substitution do you recall exactly how much water you added to the Greek yogurt?